Orbbö > Articles > Why the Microenvironment Matters in Stem Cell Treatment

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Thursday, January 5th, 2017

Effective stem cell treatment benefits from a microenvironment to focus the specific cellular activity toward an intended tissue formation. An example of this would be Rybone®.

Research has proven that effective stem cell treatment can depend on a microenvironment scaffolding that focuses on the bone tissue formation’s cellular activity. The microenvironment is also imperative to the migration of progenitor (hematopoietic and mesenchymal) cells and provides signals for the intended cellular differentiation of osteogenic cells. The intent is a product that can help facilitate this kind of cellular activity to concentrate cellular response to the intended area.

When a stem cell adheres to a scaffolding, the cell goes on to a process of differentiating into lineages toward the creation of multiple cell types which cumulatively influence the development, function, and maintenance of organs and body systems. A microenvironment contributes to a stem cell’s differentiation path and can, therefore, influence its cell lineage.

They can include components such as soluble factors, extracellular matrix proteins, signaling molecules, effects of physical forces on scaffolding, and so on; It is the surrounding of a cell that influences the cell’s behavior. A stem cell would operate and develop behaviors and characteristics that decide what type of differentiation should result within Rybone®. Microenvironments include components such as soluble factors, extracellular matrix proteins, signaling molecules, effects of physical forces on scaffolding, and so on. It is the surrounding of a cell that influences the cell’s behavior. The growth factors that are native to demineralized osteogenic material coupled with an infiltration of blood-derived growth factors brought by the reconstitution with bioactive material and supports osteogenic cellular differentiation.

The specific microenvironment of stem cells is called the stem cell niche. Nearly every tissue of the adult organism maintains a population of slow-cycling stem cells for tissue homeostasis and they can respond to injury when challenged. These cells are regulated and supported by the surrounding microenvironment. The niche includes all cellular and non-cellular components that interact to control the adult stem cell. Even forces such as compression in accordance with Wolff’s law may affect the stem cell lineage toward an osteogenic response resulting in dense bone reformation.

Rybone® is a resorbable product that has moldable and formable handling capabilities when reconstituted with a bioactive material. The scaffolding environment influences the development of stem cells from quiescence through stages of differentiation. Normal cells grown in a petri dish will divide until they completely cover the surface when they experience contact inhibition and homeostasis. Similarly, cells can grow on and within graft scaffolding to the point of homeostasis and therefore the capability to maintain fusion site integrity is important. Rybone® can stay in place as intended without being affected by carrier material qualities which can over hydrate and wash away or potentially influence the strength of a fusion due to inherent density characteristics of the carrier. Rybone® has a fibrous structure; to better adhere to adjacent bone to maintain fusion site integrity.

Cells that adhere to extracellular matrix can sense the characteristics of their surroundings when anchoring with adhesion molecules like integrins and cadherins or by pulling with myosin-based contraction which can generate small contractile forces that can change the organization of an extracellular matrix.

Rybone® is derived from cortical bone (which is the hardest part of the bone, closest to the periosteum where within the inner layer or cambium, progenitor cells and osteoblasts reside). This is intentional because scaffolding density can influence cells to reorganize their cytoskeletons and cellular processes. So, cortical characteristics can potentially further improve their rigidity.

This is a product designed to support the body’s natural healing process and is one of the only products to retain optimal handling capabilities without carrier additives.

Rybone® may also offer an advantage over synthetic grafts which potentially provide little benefit when used in conjunction with autologous whole blood, stem cell or PRP reconstitution.



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